In Vitro Antagonism with the Combination of Vancomycin and Clindamycin Against Staphylococcus aureus
نویسنده
چکیده
The activity of vancomycin in combination with clindamycin against methicillin resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococcus aureus (MSSA) was evaluated. Fractional inhibitory concentration (FIC) testing and traditional time-kill experiments were performed, with each drug alone and in combination at concentrations ranging from 0.2550x the minimum inhibitory concentration (MIC). Vancomycin and clindamycin MICs were 1, 0.25 and 1, 0.5 μg/mL, while FICs were 16 and 8 for the MRSA and MSSA strains respectively. Bacterial kill at 8 and 24 hours showed vancomycin 10, 20x-clindamycin 0.2550x MIC to be highly antagonistic. Combinations of lower vancomycin concentrations (0.25-2x MIC) and high clindamycin concentrations (10-50x MIC) resulted in antagonism, whereas low Booker-vol4,no3 1/10/70 8:07 PM Page 385
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